Dual-view photoacoustic microscopy for quantitative cell nuclear imaging.
Opt Lett
; 43(20): 4875-4878, 2018 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-30320772
ABSTRACT
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging imaging modality for studying biological tissues. However, in conventional single-view OR-PAM, the lateral and axial resolutions-determined optically and acoustically, respectively-are highly anisotropic. In this Letter, we introduce dual-view OR-PAM to improve axial resolution, achieving three-dimensional (3D) resolution isotropy. We first use 0.5 µm polystyrene beads and carbon fibers to validate the resolution isotropy improvement. Imaging of mouse brain slices further demonstrates the improved resolution isotropy, revealing the 3D structure of cell nuclei in detail, which facilitates quantitative cell nuclear analysis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Fotoacústicas
/
Microscopia
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article