Glucose-Responsiveness of Pancreatic ß-Like (GRP ß-L) Cells Generated from Human Pluripotent Stem Cells.
Curr Protoc Hum Genet
; 100(1): e71, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30335214
ABSTRACT
The International Diabetic Federation estimated that 415 million adults currently have diabetes and 318 million adults had impaired glucose tolerance, putting them at high risk of developing diabetes in the future. In Type 1 Diabetes (T1D), the ß cells are lost because of autoimmune reactions. Although islet transplantation has been a promising therapy for T1D, it is greatly limited by pancreatic donors. Here, we describe a protocol to generate glucose- responsive pancreatic ß-like (GRPß-L) cells from human-induced pluripotent stem (iPS) cells. We recapitulate in vivo pancreas development by in vitro induction of differentiating human (iPS) cells with stage-specific signaling molecules and proteins. Inhibition of Tyrosine Kinase receptor AXL, TGF-ß, and Notch signaling pathways in the final stage of the five-stage protocol could efficiently generate GRPß-L from the endocrine progenitor. Differentiation of human iPS cells through the protocol could result in functional GRPß-L cells, which could be used in pharmaceutical and ß cell biology studies. © 2018 by John Wiley & Sons, Inc.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Técnicas de Cultura de Células
/
Diabetes Mellitus Tipo 1
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Células Secretoras de Insulina
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article