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Peptide vaccine against chikungunya virus: immuno-informatics combined with molecular docking approach.
Tahir Ul Qamar, Muhammad; Bari, Amna; Adeel, Muhammad Muzammal; Maryam, Arooma; Ashfaq, Usman Ali; Du, Xiaoyong; Muneer, Iqra; Ahmad, Hafiz Ishfaq; Wang, Jia.
Afiliação
  • Tahir Ul Qamar M; Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University (HZAU), Wuhan, People's Republic of China.
  • Bari A; Department of Bioinformatics and Biotechnology, Government College University Faisalabad (GCUF), Faisalabad, Pakistan.
  • Adeel MM; Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University (HZAU), Wuhan, People's Republic of China.
  • Maryam A; Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan.
  • Ashfaq UA; Department of Bioinformatics and Biotechnology, Government College University Faisalabad (GCUF), Faisalabad, Pakistan. usmancemb@gmail.com.
  • Du X; Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University (HZAU), Wuhan, People's Republic of China.
  • Muneer I; Key Lab of Animal Genetics, Breeding and Reproduction of Ministry Education, College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
  • Ahmad HI; School of Life Sciences, University of Science and Technology of China, Hefei, People's Republic of China.
  • Wang J; Key Lab of Animal Genetics, Breeding and Reproduction of Ministry Education, College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
J Transl Med ; 16(1): 298, 2018 10 27.
Article em En | MEDLINE | ID: mdl-30368237
ABSTRACT

BACKGROUND:

Chikungunya virus (CHIKV), causes massive outbreaks of chikungunya infection in several regions of Asia, Africa and Central/South America. Being positive sense RNA virus, CHIKV replication within the host resulting in its genome mutation and led to difficulties in creation of vaccine, drugs and treatment strategies. Vector control strategy has been a gold standard to combat spreading of CHIKV infection, but to eradicate a species from the face of earth is not an easy task. Therefore, alongside vector control, there is a dire need to prevent the infection through vaccine as well as through antiviral strategies.

METHODS:

This study was designed to find out conserved B cell and T cell epitopes of CHIKV structural proteins through immuno-informatics and computational approaches, which may play an important role in evoking the immune responses against CHIKV.

RESULTS:

Several conserved cytotoxic T-lymphocyte epitopes, linear and conformational B cell epitopes were predicted for CHIKV structural polyprotein and their antigenicity was calculated. Among B-cell epitopes "PPFGAGRPGQFGDI" showed a high antigenicity score and it may be highly immunogenic. In case of T cell epitopes, MHC class I peptides 'TAECKDKNL' and MHC class II peptides 'VRYKCNCGG' were found extremely antigenic.

CONCLUSION:

The study led to the discovery of various epitopes, conserved among various strains belonging to different countries. The potential antigenic epitopes can be successfully utilized in designing novel vaccines for combating and eradication of CHIKV disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus Chikungunya / Vacinas de Subunidades Antigênicas / Simulação de Acoplamento Molecular Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus Chikungunya / Vacinas de Subunidades Antigênicas / Simulação de Acoplamento Molecular Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article