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Multi-dimensional flow cytometry analysis reveals increasing changes in the systemic neutrophil compartment during seven consecutive days of endurance exercise.
van Staveren, Selma; Ten Haaf, Twan; Klöpping, Margot; Hilvering, Bart; Tinnevelt, Gerjen H; de Ruiter, Karin; Piacentini, Maria F; Roelands, Bart; Meeusen, Romain; de Koning, Jos J; Jansen, Jeroen J; Vrisekoop, Nienke; Koenderman, Leo.
Afiliação
  • van Staveren S; Department of Respiratory Medicine, Laboratory of Translational Immunology, UMC Utrecht, Utrecht, The Netherlands.
  • Ten Haaf T; TI-COAST, Amsterdam, The Netherlands.
  • Klöpping M; Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, MOVE Research Institute Amsterdam, Amsterdam, The Netherlands.
  • Hilvering B; Department of Respiratory Medicine, Laboratory of Translational Immunology, UMC Utrecht, Utrecht, The Netherlands.
  • Tinnevelt GH; Department of Respiratory Medicine, Laboratory of Translational Immunology, UMC Utrecht, Utrecht, The Netherlands.
  • de Ruiter K; Analytical Chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands.
  • Piacentini MF; TI-COAST, Amsterdam, The Netherlands.
  • Roelands B; Department of Parasitology, LUMC Leiden, Leiden, The Netherlands.
  • Meeusen R; Department of Human Movement and Sport Sciences, University of Rome "Foro Italico", Rome, Italy.
  • de Koning JJ; Human Physiology Research Group, Vrije Universiteit Brussel, Brussels, Belgium.
  • Jansen JJ; Human Physiology Research Group, Vrije Universiteit Brussel, Brussels, Belgium.
  • Vrisekoop N; School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University, Townsville, Queensland, Australia.
  • Koenderman L; Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, MOVE Research Institute Amsterdam, Amsterdam, The Netherlands.
PLoS One ; 13(10): e0206175, 2018.
Article em En | MEDLINE | ID: mdl-30376575
ABSTRACT
Endurance exercise is associated with a transient increase in neutrophil counts in the peripheral blood. Here we investigate the impact of intensified endurance exercise on the neutrophil compartment. We hypothesized that intensified endurance exercise leads to mobilization of neutrophil subsets, which are normally absent in the blood. Furthermore, we followed the potential build-up of neutrophil activation and the impact on overnight recovery of the neutrophil compartment during a seven-day cycling tour. The neutrophil compartment was studied in 28 healthy amateur cyclists participating in an eight-day strenuous cycling tour. Blood samples were taken at baseline, after 4 days and after 7 days of cycling. The neutrophil compartment was analyzed in terms of numbers and its phenotype by deep phenotyping of flow cytometry data with the multi-dimensional analysis method FLOOD. Repeated endurance exercise led to a gradual increase in total neutrophil counts over the days leading to a 1.26 fold-increase (95%CI 1.01-1.51 p = 0.0431) in the morning of day 8. Flow cytometric measurements revealed the appearance of 2 additional neutrophil subsets CD16brightCD62Ldim and CD16dimCD62Lbright. A complex change in neutrophil phenotypes was present characterized by decreased expression of both CD11b and CD62L and marked increased expression of LAIR-1, VLA-4 and CBRM1/5. The changes in expression were found on all neutrophils present in the blood. Strikingly, in strong contrast to our findings during acute inflammation evoked by LPS challenge, these neutrophils did not upregulate classical degranulation markers. In fact, our FLOOD analysis revealed that the exercise induced neutrophil phenotype did not overlap with the neutrophil subsets arising upon acute inflammation. In conclusion, during multiple days of endurance exercise the neutrophil compartment does not regain homeostasis overnight. Thereby our study supports the concept of a build-up of inflammatory cues during repeated endurance exercise training, causing a prolonged change of the systemic neutrophil compartment.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resistência Física / Citometria de Fluxo / Neutrófilos Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resistência Física / Citometria de Fluxo / Neutrófilos Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2018 Tipo de documento: Article