Hydrophilic interaction liquid chromatography coupled with quadrupole-orbitrap ultra high resolution mass spectrometry to quantitate nucleobases, nucleosides, and nucleotides during white tea withering process.
Food Chem
; 266: 343-349, 2018 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-30381196
ABSTRACT
Nucleotides, nucleosides, and nucleobases are important bioactive compounds. Recent studies suggested that they possess taste activity. However, it remains unknown about their presence in white tea and how they change during white tea manufacture. Here, we first established method based on hydrophilic interaction liquid chromatography coupled with quadrupole-orbitrap ultra high resolution mass spectrometry (HILIC-Quadrupole-Orbitrap-UHRMS) platform, then applied it to study the dynamic changes of nucleotides, nucleosides, and nucleobases during white tea withering process. Five compounds, including adenosine 5'-monophosphate monohydrate (AMP), guanosine 5'-monophosphate disodium salt hydrate (GMP), adenosine, cytidine, thymine and uracil, were detected from withering samples. They showed a general decline trend during white tea withering process, however, considerable amount of them was retained after withering for 48â¯h except adenosine which was below detection limit after withering for 21â¯h. This study provided a complete picture about nucleotides, nucleosides and nucleobases changes during white tea withering process.
Palavras-chave
Adenine (PubChem CID: 190); Adenosine (PubChem CID: 60961); Adenosine 5'-monophosphate monohydrate (AMP) (PubChem CID: 6083); Cytidine (PubChem CID: 6175); Cytidine 5'-monophosphate (CMP) (PubChem CID: 6131); Cytosine (PubChem CID: 597); Guanine (PubChem CID: 764); Guanosine (PubChem CID: 6802); Guanosine 5'-monophosphate disodium salt hydrate (GMP) (PubChem CID: 21712); HILIC-Quadrupole-Orbitrap-UHRMS; Hypoxanthine (PubChem CID: 790); Inosine (PubChem CID: 6021); Inosine-5'-monophosphate disodium salt octahydrate (IMP) (PubChem CID: 8582); Nucleobases; Nucleosides; Nucleotides; Thymine (PubChem CID: 1135); Uracil (PubChem CID: 1174); Uridine (PubChem CID: 6029); Uridine 5'-monophosphate disodium salt (UMP) (PubChem CID: 6030); White tea; Withering process; Xanthine (PubChem CID: 1188)
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
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Chá
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Cromatografia Líquida
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Interações Hidrofóbicas e Hidrofílicas
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Manipulação de Alimentos
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Nucleosídeos
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Nucleotídeos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article