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Biological control of the soft rot bacterium Pectobacterium carotovorum by Bacillus amyloliquefaciens strain Ar10 producing glycolipid-like compounds.
Azaiez, Sana; Ben Slimene, Imen; Karkouch, Ines; Essid, Rym; Jallouli, Selim; Djebali, Naceur; Elkahoui, Salem; Limam, Ferid; Tabbene, Olfa.
Afiliação
  • Azaiez S; Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-lif, Tunisia; Université Tunis El Manar, Campus Universitaire Farhat Hached, BP-94 Rommana, 1068 Tunis, Tunisia.
  • Ben Slimene I; Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-lif, Tunisia.
  • Karkouch I; Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-lif, Tunisia.
  • Essid R; Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-lif, Tunisia.
  • Jallouli S; Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-lif, Tunisia.
  • Djebali N; Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-lif, Tunisia.
  • Elkahoui S; Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-lif, Tunisia.
  • Limam F; Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-lif, Tunisia.
  • Tabbene O; Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-lif, Tunisia. Electronic address: tabb_olfa@yahoo.fr.
Microbiol Res ; 217: 23-33, 2018 Dec.
Article em En | MEDLINE | ID: mdl-30384906
Four hundred and fifty bacteria were evaluated for antagonistic activity against bacterial soft rot of potato caused by Pectobacterium carotovorum sp strain II16. A strain Ar10 exhibiting potent antagonist activity has been identified as Bacillus amyloliquefaciens on the basis of biochemical and molecular characterization. Cell free supernatant showed a broad spectrum of antibacterial activity against human and phytopathogenic bacteria in the range of 10-60 AU/mL. Incubation of P. carotovorum cells with increasing concentrations of the antibacterial compound showed a killing rate of 94.8 and 96% at MIC and 2xMIC respectively. In addition, the antibacterial agent did not exert haemolytic activity at the active concentration and has been preliminary characterized by TLC and GC-MS as a glycolipid compound. Treatment of potato tubers with strain Ar10 for 72 h significantly reduced the severity of disease symptoms (100 and 85.05% reduction of necrosis deep / area and weight loss respectively). The same levels in disease symptoms severity was also recorded following treatment of potato tubers with cell free supernatant for 1 h. Data suggest that protection against potato soft rot disease may be related to glycolipid production by strain Ar10. The present study affords new alternatives for anti-Pectobacterium carotovorum bioactive compounds against the soft rot disease of potato.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Glicolipídeos / Pectobacterium carotovorum / Agentes de Controle Biológico / Bacillus amyloliquefaciens / Antibacterianos Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Glicolipídeos / Pectobacterium carotovorum / Agentes de Controle Biológico / Bacillus amyloliquefaciens / Antibacterianos Idioma: En Ano de publicação: 2018 Tipo de documento: Article