A fluorescent sensor for discrimination of HSA from BSA through selectivity evolution.
Anal Chim Acta
; 1043: 123-131, 2018 Dec 28.
Article
em En
| MEDLINE
| ID: mdl-30392660
ABSTRACT
Pre-clinical diagnosis of many diseases required quantitative detection of Human Serum Albumin (HSA). Herein a high-selective HSA sensor RhHSA was picked through a two-round selectivity evolution from the typical "Effector-π-Trigger" style. RhHSA suggested advantages including high selective (â¼6 fold for HSABSAâ¯=â¯110), sensitive (LODâ¯â¼â¯5â¯nM, over 700-fold enhancement), steady (over 24â¯h) and wide linear range (0-0.5â¯mg/mL, applicative for conventional HSA measurement). The detecting system was free from media polarity or viscosity. HSA destruction, site competition and molecular docking provided reliable evidence for the fact that RhHSA could be embedded into both ibuprofen and phenylbutazone sites of HSA. These hints also supported the discrimination of HSA from BSA. Stepwisely fluid replacement in living cells and measuring in urine system both inferred the potential of RhHSA in biological applications.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Soroalbumina Bovina
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Corantes Fluorescentes
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Albumina Sérica Humana
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article