Adipose tissue-derived mesenchymal stem cells cultured at high density express IFN-ß and TRAIL and suppress the growth of H460 human lung cancer cells.
Cancer Lett
; 440-441: 202-210, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30393160
ABSTRACT
Although mesenchymal stem cells (MSCs) have been reported to inhibit tumor growth, the mechanism controlling this tumor suppression function is unclear. Here, we report that high-density (40,000â¯cells/cm2) cultured adipose tissue-derived MSCs (40K-ASCs) expressed interferon (IFN)-ß and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL); we also found that serum deprivation during cell culture induced the expression of IFN-ß and TRAIL. In addition, the mRNA expression of IFN-ß, but not TRAIL, was increased during the washing step required for the transplantation of normal-density (5000â¯cells/cm2) cultured ASCs (5K-ASCs). When the human lung cancer cell line H460 was co-cultured with 40K-ASCs, necrotic cell death was dramatically increased in vitro. When ASCs were injected after four washes, both 5K-ASCs and 40K-ASCs substantially reduced tumor weight in H460-derived cancer animal models. These results suggest that serum deprivation during the culture of 40K-ASCs or during the washing step of 5K-ASCs can induce IFN-ß and/or TRAIL expression, ultimately leading to the tumor suppression capability of ASCs.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interferon beta
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Ligante Indutor de Apoptose Relacionado a TNF
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Células-Tronco Mesenquimais
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Neoplasias Pulmonares
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Animals
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Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article