Fast-scanning photoacoustic microscopy with a side-looking fiber optic ultrasound sensor.
Biomed Opt Express
; 9(11): 5809-5816, 2018 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-30460163
ABSTRACT
Optical-resolution photoacoustic microscopy (OR-PAM) images biological tissue with sub-cellular resolution and optical absorption contrast. OR-PAM is limited by the tradeoff among imaging speed, field of view, and sensitivity. In this work, we present an OR-PAM technique based on an unfocused side-looking fiber optic ultrasound (FOUS) sensor, which achieves high imaging speed, large field of view, and good sensitivity for in vivo imaging. The FOUS sensor is developed based on a dual-polarized fiber laser and read out with real-time frequency demodulation. Via minimizing the readout noise, the sensor offers a noise-equivalent pressure of 43.6 Pa, enabling high detection sensitivity over a large field of view. High imaging speed is achieved via scanning the laser beam with a 2D galvo mirror in the ultrasound detection area. Microvascular imaging with a frame rate of 2 Hz over a 2 × 2 mm2 area has been demonstrated in the mouse ear. The new OR-PAM technique may be used in the visualization of biological and physiologic dynamics.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article