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Induction of chronic destructive arthritis in SCID mice by arthritogenic fibroblast-like synoviocytes derived from mice with antigen-induced arthritis.
Frey, Oliver; Hückel, Marion; Gajda, Mieczyslaw; Petrow, Peter K; Bräuer, Rolf.
Afiliação
  • Frey O; Institute of Pathology, University Hospital, Jena, Germany. o.frey@imd-berlin.de.
  • Hückel M; Institute of Clinical Chemistry and Laboratory Medicine, University Hospital, Am Klinikum 1, D-07743, Jena, Germany. o.frey@imd-berlin.de.
  • Gajda M; Present address: Institute of Medical Diagnostics, Berlin, Germany. o.frey@imd-berlin.de.
  • Petrow PK; Institute of Pathology, University Hospital, Jena, Germany.
  • Bräuer R; Institute of Pathology, University Hospital, Jena, Germany.
Arthritis Res Ther ; 20(1): 261, 2018 11 22.
Article em En | MEDLINE | ID: mdl-30466479
BACKGROUND: Fibroblast-like synoviocytes (FLSs) from patients with rheumatoid arthritis (RA) are autonomously activated to maintain inflammation and joint destruction in co-transplantation models. To elucidate inducing mechanisms involved in this altered behavior, the arthritogenic potential of FLSs from murine antigen-induced arthritis (AIA) were investigated in a transfer model. METHODS: FLSs were isolated, expanded in vitro, and transferred into knee joint cavities of severe combined immunodeficient (SCID) mice. Their arthritogenic capacity was assessed by monitoring joint swelling and evaluation of histological parameters 70 to 100 days after transfer. RESULTS: FLSs from AIA mice were able to transfer arthritis into recipient SCID mice. FLS transfer induced a chronic arthritis with recruitment of inflammatory cells and marked cartilage destruction. Long-lasting inflammation was not required for imprinting of arthritogenicity in FLSs since cells isolated from acute arthritic joints were fully competent to transfer arthritis. We also observed arthritogenic potential in FLSs isolated from contralateral non-arthritic joints in our monoarticular arthritis model. CONCLUSIONS: We show that the transformation of FLSs into arthritogenic cells occurs early in arthritis development. This challenges current hypotheses on the role of these cells in arthritis pathogenesis and opens up the way for further mechanistic studies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artrite Experimental / Artrite Reumatoide / Fibroblastos / Sinoviócitos / Articulação do Joelho Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artrite Experimental / Artrite Reumatoide / Fibroblastos / Sinoviócitos / Articulação do Joelho Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article