Substrate Elasticity Governs Differentiation of Renal Tubule Cells in Prolonged Culture.
Tissue Eng Part A
; 25(13-14): 1013-1022, 2019 07.
Article
em En
| MEDLINE
| ID: mdl-30484388
ABSTRACT
IMPACT STATEMENT Successful clinical tissue engineering requires functional fidelity of the cultured cell to its in vivo counterpart, but this has been elusive in renal tissue engineering. Typically, renal proximal tubule cells in culture have a flattened morphology and do not express key transporters essential to their function. In this article, we show for the first time that in vitro substrate mechanical properties dictate differentiation of cultured renal proximal tubule cells. Remarkably, this effect was only discernable after 4 weeks in culture, longer than usually reported for this cell type. These results demonstrate a new tunable parameter to optimize cell differentiation in renal tissue engineering.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Hidrogéis
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Elasticidade
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Túbulos Renais
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article