In-Cell NMR Spectroscopy of Intrinsically Disordered Proteins.

Sciolino, Nicholas; Burz, David S; Shekhtman, Alexander

Sciolino, Nicholas; Burz, David S; Shekhtman, Alexander.

Afiliação

Sciolino N; Department of Chemistry, University at Albany, State University of New York, Albany, NY, 12222, USA.
Burz DS; Department of Chemistry, University at Albany, State University of New York, Albany, NY, 12222, USA.
Shekhtman A; Department of Chemistry, University at Albany, State University of New York, Albany, NY, 12222, USA.

Proteomics ; 19(6): e1800055, 2019 03.

Article em En | MEDLINE | ID: mdl-30489014

RESUMO

This review summarizes the results of in-cell Nuclear Magnetic Resonance, NMR, spectroscopic investigations of the eukaryotic and prokaryotic intrinsically disordered proteins, IDPs: α-synuclein, prokaryotic ubiquitin-like protein, Pup, tubulin-related neuronal protein, Tau, phenylalanyl-glycyl-repeat-rich nucleoporins, FG Nups, and the negative regulator of flagellin synthesis, FlgM. The results show that the cellular behavior of IDPs may differ significantly from that observed in the test tube.

Assuntos

Proteínas Intrinsicamente Desordenadas/metabolismo; Ressonância Magnética Nuclear Biomolecular/métodos; Animais; Humanos; Proteínas Intrinsicamente Desordenadas/química; Modelos Moleculares; Conformação Proteica; Mapeamento de Interação de Proteínas/métodos

Palavras-chave

Mycobacterium tuberculosis; NMR spectroscopy; in-cell NMR; protein-protein interactions; synuclein

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ressonância Magnética Nuclear Biomolecular / Proteínas Intrinsicamente Desordenadas Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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