Stereoselective catalysis controlled by a native leucine or variant isoleucine wing-gatekeeper in 2-haloacid dehalogenase.

Feng, Yanbin; Wang, Yayue; Chu, Huiying; Fan, Yan; Cao, Xupeng; Liu, Yinghui; Li, Guohui; Xue, Song

Feng, Yanbin; Wang, Yayue; Chu, Huiying; Fan, Yan; Cao, Xupeng; Liu, Yinghui; Li, Guohui; Xue, Song.

Afiliação

Feng Y; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Wang Y; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Chu H; School of Biology and Food Science, Shangqiu Normal University, China.
Fan Y; Laboratory of Molecular Modeling and Design, State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, China.
Cao X; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Liu Y; University of Chinese Academy of Sciences, Beijing, China.
Li G; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Xue S; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

FEBS Lett ; 593(3): 308-318, 2019 02.

Article em En | MEDLINE | ID: mdl-30499103

Assuntos

Proteínas de Bactérias/química; Hidrolases/química; Pseudomonas putida/enzimologia; Proteínas de Bactérias/genética; Proteínas de Bactérias/metabolismo; Catálise; Hidrolases/genética; Hidrolases/metabolismo; Isoleucina/química; Isoleucina/genética; Isoleucina/metabolismo; Leucina/química; Leucina/genética; Leucina/metabolismo; Pseudomonas putida/genética

Palavras-chave

biocatalyst; enzyme; molecular dynamics; protein engineering; stereoselectivity

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Pseudomonas putida / Hidrolases Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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