The expression of EMX2 lead to cell cycle arrest in glioblastoma cell line.
BMC Cancer
; 18(1): 1213, 2018 Dec 04.
Article
em En
| MEDLINE
| ID: mdl-30514244
ABSTRACT
BACKGROUND:
Glioblastoma (GB) is a highly invasive primary brain tumor that nearly always systematically recurs at the site of resection despite aggressive radio-chemotherapy. Previously, we reported a gene expression signature related to tumor infiltration. Within this signature, the EMX2 gene encodes a homeodomain transcription factor that we found was down regulated in glioblastoma. As EMX2 is reported to play a role in carcinogenesis, we investigated the impact of EMX2 overexpression in glioma-related cell lines.METHODS:
For that purpose, we constructed tetracycline-inducible EMX2 expression lines. Transfected cell phenotypes (proliferation, cell death and cell cycle) were assessed in time-course experiments.RESULTS:
Restoration of EMX2 expression in U87 glioblastoma cells significantly inhibited cell proliferation. This inhibition was reversible after EMX2 removal from cells. EMX2-induced proliferative inhibition was very likely due to cell cycle arrest in G1/S transition and was not accompanied by signs of cell death.CONCLUSION:
Our results suggest that EMX2 may constitute a putative therapeutic target for GB treatment. Further studies are required to decipher the gene networks and transduction signals involved in EMX2's effect on cell proliferation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Neoplasias Encefálicas
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Regulação Neoplásica da Expressão Gênica
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Glioblastoma
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Proteínas de Homeodomínio
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Pontos de Checagem do Ciclo Celular
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article