Physicochemical properties of polysaccharides separated from Camellia oleifera Abel seed cake and its hypoglycemic activity on streptozotocin-induced diabetic mice.
Int J Biol Macromol
; 125: 1075-1083, 2019 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-30529353
ABSTRACT
Two homogeneous polysaccharide fractions named SCP-1 (7.16â¯×â¯106â¯Da) and SCP-2 (2.00â¯×â¯104â¯Da) were purified by DEAE-52 cellulose and Sephadex G-200 column chromatography successively from Camellia oleifera Abel seed cake. They were characterized by Fourier transformed infrared (FT-IR), high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC). The monosaccharide compositions of SCP-1 were dmannose, dglucose and lxylose with a molar ratio of 1.770.931 and that of the SCP-2 were dmannose, lrhamnose, dglucose and lxylose with a molar ratio of 5.271.210.161. Animal experiments suggested that the plasma glucose levels in hyperglycemia mice were reduced by 11.34%, 30.70%, 46.83% after administration of high, medium and low doses of SCP-1, and reduced by 16.67%, 23.93% and 33.00% after administration of high, medium and low doses of SCP-2, respectively. SCP-1 and SCP-2 also increased the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), as well as decreased the content of malondialdehyde (MDA) in the hyperglycemia mice. These results suggested that SCP-1 and SCP-2 possessed strong hypoglycemic activities in streptozotocin-induced model mice. In addition, the hypoglycemic activity of SCP-1 was stronger than that of SCP-2.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Camellia
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Diabetes Mellitus Experimental
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Hiperlipidemias
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Hipoglicemiantes
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Antioxidantes
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article