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Isolation and culture of human primary keratinocytes-a methods review.
Sciezynska, Aneta; Nogowska, Aleksandra; Sikorska, Maria; Konys, Justyna; Karpinska, Anita; Komorowski, Michal; Oldak, Monika; Malejczyk, Jacek.
Afiliação
  • Sciezynska A; Department of Histology and Embryology, Medical University of Warsaw, Warsaw, Poland.
  • Nogowska A; Department of Regenerative Medicine and Cell Biology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
  • Sikorska M; Department of Histology and Embryology, Medical University of Warsaw, Warsaw, Poland.
  • Konys J; Department of Immunology, Biochemistry and Nutrition, Medical University of Warsaw, Warsaw, Poland.
  • Karpinska A; Laboratory for Cell Research and Application, Medical University of Warsaw, Warsaw, Poland.
  • Komorowski M; Department of Histology and Embryology, Medical University of Warsaw, Warsaw, Poland.
  • Oldak M; Department of Regenerative Medicine and Cell Biology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
  • Malejczyk J; Department of Regenerative Medicine and Cell Biology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
Exp Dermatol ; 28(2): 107-112, 2019 02.
Article em En | MEDLINE | ID: mdl-30548893
Keratinocyte culture is a necessary and widely used tool in a variety of experimental dermatological and biomedical studies. Literature search has revealed a variety of different protocols of human primary keratinocyte isolation and culture. Therefore, the aim of this paper was to review and summarize current trends in human primary keratinocyte culture techniques. We present data on the most popular and effective methods of human keratinocyte isolation and cultivation obtained from screening of 945 papers published during the last 10 years.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Queratinócitos / Técnicas de Cultura de Células Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Queratinócitos / Técnicas de Cultura de Células Limite: Animals / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article