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Achyranthes bidentata polypeptides promotes migration of Schwann cells via NOX4/DUOX2-dependent ROS production in rats.
Song, Honghua; Zhao, Hualong; Yang, Lingyan; Li, Lilan; Zhang, Tuanjie; Pan, Jiacheng; Meng, Yali; Shen, Weixing; Yuan, Ying.
Afiliação
  • Song H; Department of Pediatrics, Affiliated Hospital of Nantong University, Nantong, 226001, PR China. Electronic address: song_hong_hua@126.com.
  • Zhao H; Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: zhaohualong2011@163.com.
  • Yang L; Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: 124152828@qq.com.
  • Li L; Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: 1152280227@qq.com.
  • Zhang T; Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: 383761825@qq.com.
  • Pan J; Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: kaixinge@126.com.
  • Meng Y; Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: 965048703@qq.com.
  • Shen W; Medical School, Nantong University, Nantong, 226001, PR China. Electronic address: swx@ntu.edu.cn.
  • Yuan Y; Department of Personnel, Affiliated Hospital of Nantong University, Nantong, 226001, PR China; Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: yuanying@ntu.edu.cn.
Neurosci Lett ; 696: 99-107, 2019 03 23.
Article em En | MEDLINE | ID: mdl-30572102
Achyranthes bidentata polypeptides (ABPP), an active polypeptides isolated from the aqueous extract of Achyranthes bidentata Blume, contributes to the regeneration of injured peripheral nerves by promoting migration of Schwann cells (SCs). In this study, we aimed to investigate the possible mechanism underlying the ABPP-induced migration of primary cultured rat SCs. Transwell migration assays indicated that ABPP promoted SCs migration in a concentration-dependent manner by inducing production of NADPH-oxidase (NOX)-derived reactive oxygen species (ROS). Inhibition of ROS production by NOXs inhibitor apocynin (APO) or diphenyleneiodonium (DPI) partially blocked ABPP-mediated SCs migration. Furthermore, by using real-time polymerase chain reaction analysis and siRNA interference technique, we verified the participation of NOX subunit 4 (NOX4) and dual oxidase 2 (DUOX2) in ABPP-induced ROS production and consequential SCs migration. Taken together, these results demonstrated that ABPP promoted SCs migration via NOX4/DUOX2-activated ROS in SCs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Células de Schwann / Espécies Reativas de Oxigênio / Fármacos Neuroprotetores Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Células de Schwann / Espécies Reativas de Oxigênio / Fármacos Neuroprotetores Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article