LncRNA TUG1 promotes osteoarthritis-induced degradation of chondrocyte extracellular matrix via miR-195/MMP-13 axis.
Eur Rev Med Pharmacol Sci
; 22(24): 8574-8581, 2018 12.
Article
em En
| MEDLINE
| ID: mdl-30575896
OBJECTIVE: Osteoarthritis is a degenerative disease characterized by articular cartilage degradation. Long non-coding ribonucleic acid (lncRNA) plays important roles in a series of biological processes, but its role in osteoarthritis is still not quite clear. This study aims to investigate the regulatory role of taurine upregulated gene 1 (TUG1) in osteoarthritis. PATIENTS AND METHODS: The expression level of lncRNA-TUG1 in cartilages of patients with osteoarthritis and those of normal people was compared using Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Primary chondrocytes were induced by interleukin-1 beta (IL-1ß) and tumor necrosis factor-alpha (TNF-α), followed by expression detection of lncRNA-TUG1, microRNA-195 (miR-195), and matrix metalloproteinase-13 (MMP-13). In addition, in vitro regulatory roles of lncRNA-TUG1 and miR-195 in osteoarthritis were verified by transfection of lncRNA-TUG1 and miR-195 plasmids. The dimethylmethylene blue (DMMB) assay was performed to analyze the secretion and formation of soluble sulfated glycosaminoglycan (sGAG). RESULTS: The expression levels of lncRNA-TUG1 and MMP-13 in cartilages of patients with osteoarthritis were higher than those in cartilages of normal people, while the level of miR-195 decreased in cartilages of patients with osteoarthritis. After chondrocytes were induced by IL-1ß and TNF-α, the expression of lncRNA-TUG1 increased. Overexpression of lncRNA-TUG1 decreased the expressions of miR-195, collagen, and aggrecan, but increased the expression of MMP-13. LncRNA-TUG1 knockdown obtained the opposite results. CONCLUSIONS: LncRNA-TUG1 regulates the degradation of extracellular matrix in osteoarthritis via lncRNA-TUG1/miR-195/MMP-13 axis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoartrite
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Condrócitos
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MicroRNAs
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Matriz Extracelular
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Metaloproteinase 13 da Matriz
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RNA Longo não Codificante
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article