Morphokinetic parameters in chromosomal translocation carriers undergoing preimplantation genetic testing.
Reprod Biomed Online
; 38(2): 177-183, 2019 Feb.
Article
em En
| MEDLINE
| ID: mdl-30579822
ABSTRACT
RESEARCH QUESTION Can embryo morphokinetic parameters help identify unbalanced embryos in translocation carriers? DESIGN:
This retrospective study was conducted in 67 translocation carriers undergoing 105 preimplantation genetic testing cycles for chromosomal structural rearrangements (PGT-SR) without aneuploidy screening (PGT-A). Using time-lapse imaging analysis, morphokinetic parameters of balanced and unbalanced embryos were compared, as well as the frequency of abnormal cellular events. The performance of a previously published prediction model of aneuploidy was also tested in this population.RESULTS:
Significant differences were observed between balanced and unbalanced embryos for some morphokinetic parameters t5 (Pâ¯=â¯0.0067), t9+ (Pâ¯=â¯0.0077), cc2 (Pâ¯=â¯0.0144), s2 (Pâ¯=â¯0.0003) and t5-t2 (Pâ¯=â¯0.0028). Also, multinucleation at the two- or four-cell stages, abnormal division and cell exclusion at the morula stage were significantly (all P < 0.05) more frequent in unbalanced than in balanced embryos. None, however, could accurately predict embryo chromosomal status. A previously published morphokinetic prediction model for embryo aneuploidy did not adequately classify balanced and unbalanced embryos.CONCLUSIONS:
No significant morphokinetic predictor of chromosomal status could be found. Time-lapse should not be used as a diagnostic tool for chromosomal status in translocation carriers.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Translocação Genética
/
Aberrações Cromossômicas
/
Diagnóstico Pré-Implantação
/
Desenvolvimento Embrionário
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article