The use of clade-specific PCR assays to identify novel nitrilase genes from environmental isolates.
Microbiologyopen
; 8(4): e00700, 2019 04.
Article
em En
| MEDLINE
| ID: mdl-30597773
ABSTRACT
Nitrilase enzymes (EC 3.5.5.1) are responsible for the direct hydration of nitriles to their corresponding carboxylic acids and ammonia. The utilization of nitrilase enzymes in biocatalysis toward bio-pharmaceuticals and industrial applications facilitates the move towards green chemistry. The body of research presented describes a novel clade-specific touchdown PCR protocol for the detection of novel nitrilase genes. The presented study identified partial sequences of 15 novel nitrilase genes across 7 genera, with partial DNA sequence homology (%) displayed across an additional 16 genera. This research will prove valuable in the screening of microorganisms for the identification of novel clade-specific nitrilase genes, with predicted enantioselective profiles as determined by their clade characterizations.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Proteínas de Bactérias
/
Reação em Cadeia da Polimerase
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Microbiologia Ambiental
/
Hidroliases
Tipo de estudo:
Evaluation_studies
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article