Chemical and Topographical Single-Cell Imaging by Near-Field Desorption Mass Spectrometry.
Angew Chem Int Ed Engl
; 58(14): 4541-4546, 2019 03 26.
Article
em En
| MEDLINE
| ID: mdl-30600882
Simultaneously acquiring chemical and topographical information within a single cell at nanoscale resolutions is vital to cellular biology, yet it remains a great challenge due to limited lateral resolutions and detection sensitivities. Herein, the development of near-field desorption mass spectrometry for correlated chemical and topographical imaging is reported, thereby bridging the gap between laser-based mass spectrometry (MS) methods and multimodal single-cell imaging. Using this integrated platform, an imaging resolution of 250â
nm and 3D topographically reconstructed chemical single-cell imaging were achieved. This technique offers more in-depth cellular information than micrometer-range laser-based MS imaging methods. Considering the simplicity and compact size of the near-field device, this technique can be introduced to MALDI-MS, expanding the multimodal abilities of MS at nanoscale resolutions.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise de Célula Única
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article