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Utilization of atmospheric solids analysis probe mass spectrometry for analysis of fatty acids on seed surface.
Cechová, Monika; Hradilová, Iveta; Smýkal, Petr; Barták, Petr; Bednár, Petr.
Afiliação
  • Cechová M; Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. Listopadu 12, 771 46, Olomouc, Czech Republic.
  • Hradilová I; Department of Botany, Faculty of Science, Palacký University, Slechtitelu 27, 783 71, Olomouc, Czech Republic.
  • Smýkal P; Department of Botany, Faculty of Science, Palacký University, Slechtitelu 27, 783 71, Olomouc, Czech Republic.
  • Barták P; Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. Listopadu 12, 771 46, Olomouc, Czech Republic.
  • Bednár P; Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. Listopadu 12, 771 46, Olomouc, Czech Republic. petr.bednar@upol.cz.
Anal Bioanal Chem ; 411(6): 1169-1180, 2019 Feb.
Article em En | MEDLINE | ID: mdl-30617396
ABSTRACT
Atmospheric solids analysis probe mass spectrometry (ASAP-MS) was used for the first time for direct surface analysis of plant material. It can be readily used for surface analysis of whole and intact pea seeds and their seed coats, and for the study of the profile of fatty acids on the outer surface. Furthermore, ASAP-MS in combination with multivariate statistics allowed classification of pea genotypes with respect to physical dormancy and investigation of related biological markers. Hexacosanoic and octacosanoic acids were suggested to be important markers likely influencing water transport through the seed coat into the embryo (with the highest significance for dormant L100 genotype). ASAP-MS provided higher selectivity and better signal of fatty acids compared to (MA)LDI-MS (laser desorption ionization mass spectrometry either matrix free or matrix assisted) providing on the other hand spatial distribution information and results obtained by both methods are mutually supportive. The developed ASAP-MS method and obtained results can be widely utilized in biological, food, and agricultural research. Graphical abstract ᅟ.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sementes / Espectrometria de Massas / Pisum sativum / Ácidos Graxos Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sementes / Espectrometria de Massas / Pisum sativum / Ácidos Graxos Idioma: En Ano de publicação: 2019 Tipo de documento: Article