Host microRNA analysis in cyprinid Herpesvirus-3 (CyHV-3) infected common carp.
BMC Genomics
; 20(1): 46, 2019 Jan 16.
Article
em En
| MEDLINE
| ID: mdl-30654758
ABSTRACT
BACKGROUND:
The mechanism of latency and the ability of the cyprinid herpesvirus 3 (CyHV-3) to establish life-long infections in carp remains poorly understood. To explain the role of miRNAs in this process we applied a range of molecular tools including high-throughput sequencing of RNA libraries constructed from the blood samples of infected fish followed by bioinformatic and functional analyses which show that CyHV-3 profoundly influences the expression of host miRNAs in vivo.RESULTS:
We demonstrated the changed expression of 27 miRNAs in the clinical phase and 5 in the latent phase of infection. We also identified 23 novel, not previously reported sequences, from which 8 showed altered expressions in control phase, 10 in clinical phase and 5 in latent phase of infection.CONCLUSIONS:
The results of our analysis expand the knowledge of common carp microRNAs engaged during CyHV-3 infection and provide a useful basis for the further study of the mechanism of CyHV-3 induced pathology.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carpas
/
Infecções por Herpesviridae
/
MicroRNAs
/
Interações Hospedeiro-Patógeno
/
Doenças dos Peixes
/
Herpesviridae
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article