Expression and functional analysis of Brucella outer membrane protein 25 in recombinant goat pox virus.
Mol Med Rep
; 19(3): 2323-2329, 2019 Mar.
Article
em En
| MEDLINE
| ID: mdl-30664205
ABSTRACT
The Capripoxvirus (CaPV) has a large doublestranded DNA genome and a restricted hostrange. At present, it is being investigated as an ideal vaccine vector. In the present study, a novel recombinant goat pox virus (rGTPV) was constructed to express Brucella outer membrane protein (OMP)25, and was validated by in vitro and in vivo immunization assays. A novel rGTPV vector was created, in which the thymidine kinase gene was used as a flanking sequence, I1L was inserted as a promoter element to enhance Brucella OMP25 expression, and p7.5 as another promoter element was used to regulate guanine phosphoribosyltransferase as a selection maker. The rGTPV vector was transfected into sheep fetal fibroblast/lamb testis cells preinfected with GTPV G14STV4455 to recombine. Brucella OMP25 protein was expressed in cells by rGTPV, and activated immune reactivity to Brucella OMP25 protein, as detected by western blotting. Furthermore, rGTPV elicited, antiBrucellaspecific immunoglobulin G responses, as determined by ELISA. Mice vaccinated with rGTPV did not exhibit pathology alterations in the kidney and liver. These results suggested that the novel rGTPV was able to efï¬ciently drive Brucella OMP25 protein expression and activate immune reactivity, and may have applications in CaPV live vector vaccines and associated research.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Brucella
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Vacina contra Brucelose
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Capripoxvirus
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article