Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections.
Methods Mol Biol
; 1920: 183-218, 2019.
Article
em En
| MEDLINE
| ID: mdl-30737693
ABSTRACT
Analysis of gene (mRNA and protein) expression patterns is central to the study of embryonic development. This chapter details methods for detecting mRNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imuno-Histoquímica
/
Expressão Gênica
/
Desenvolvimento Embrionário
/
Embrião de Mamíferos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article