Visualizing the Balbiani Body in Zebrafish Oocytes.
Methods Mol Biol
; 1920: 277-293, 2019.
Article
em En
| MEDLINE
| ID: mdl-30737697
ABSTRACT
Approaches to visualize the Balbiani body of zebrafish primary oocytes using protein, RNA, and mitochondrial markers are described. The method involves isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body development in early oocytes.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Oogênese
/
Peixe-Zebra
/
Imagem Molecular
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article