Combination of ssDNA recombineering and CRISPR-Cas9 for Pseudomonas putida KT2440 genome editing.

Wu, Zhixin; Chen, Zhongqiu; Gao, Xinyue; Li, Jing; Shang, Guangdong

Wu, Zhixin; Chen, Zhongqiu; Gao, Xinyue; Li, Jing; Shang, Guangdong.

Afiliação

Wu Z; Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China.
Chen Z; Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China.
Gao X; Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China.
Li J; Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China.
Shang G; Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China. shanggd@hotmail.com.

Appl Microbiol Biotechnol ; 103(6): 2783-2795, 2019 Mar.

Article em En | MEDLINE | ID: mdl-30762073

Assuntos

Sistemas CRISPR-Cas; DNA de Cadeia Simples/genética; Edição de Genes/métodos; Genoma Bacteriano; Pseudomonas putida/genética; Biotecnologia; Proteína 9 Associada à CRISPR/metabolismo; Deleção de Genes; Plasmídeos/genética; Mutação Puntual

Palavras-chave

CRISPR-Cas9; Genome editing; Pseudomonas putida KT2440; ssDNA recombineering

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA de Cadeia Simples / Pseudomonas putida / Genoma Bacteriano / Sistemas CRISPR-Cas / Edição de Genes Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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