A transcriptomic (RNA-seq) analysis of genes responsive to both cadmium and arsenic stress in rice root.

ABSTRACT

Cadmium (Cd) and arsenic (As) are nonessential and toxic elements in rice that often occur together in contaminated paddy field soils. To understand whether rice has a common molecular response mechanism against Cd and As toxicity, 30-day seedlings (Oryza sativa L. indica) were exposed separately to Cd and As3+ in hydroponic cultures for up to 7 days. Root transcriptomic analysis of plants exposed to Cd and As for 3 days revealed that a total of 2224 genes in rice roots responded to Cd stress, while 1503 genes responded to As stress. Of these, 841 genes responded to both stressors. The genes in common to Cd and As stress were associated with redox control, stress response, transcriptional regulation, transmembrane transport, signal transduction, as well as biosynthesis and metabolism of macromolecules and sulfur compounds. In plants exposed to Cd and As separately or in combination for 3 and 7 days, qRT-PCR verification revealed that the glutathione metabolism associated gene Os09g0367700 was significantly up-regulated with respect to unexposed controls and had a positive synergistic effect under combined Cd and As stress. In addition, the redox control related genes Os06g0216000, Os07g0638300 and Os01g0294500, the glutathione metabolism related gene Os01g0530900, the cell wall biogenesis related genes Os05g0247800, Os11g0592000 and Os03g0416200, the expression regulation related genes Os07g0597200 and Os02g0168200, and the transmembrane transport related genes Os04g0524500, also varied significantly with respect to an unexposed control and displayed synergistic effects after 7 days of simultaneous exposure to Cd and As. Our identification of a novel set of genes in rice which responded to both Cd and As3+ stress may be of value in mitigating the toxicity of co-contaminated soils. These results also provide a deeper understanding of the molecular mechanisms involved in response to multi-metal/loids stress, and may be used in the genetic improvement of rice varieties.