Fabrication of N-doped multidimensional carbon nanofibers for high-performance cortisol biosensors.
Biosens Bioelectron
; 131: 30-36, 2019 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-30802785
ABSTRACT
Cortisol is an hormone that regulates blood pressure, glucose levels and carbohydrate metabolism in humans. Abnormal secretion of cortisol can cause various symptoms closely linked to psychological and physical health. In this study, high-performance field-effect transistor (FET)-based biosensors for cortisol detection were fabricated from N-doped multidimensional carbon nanofibers. Nanofiber morphology was controlled by tailoring the pressure conditions during vapor deposition polymerization (VDP). Thereafter, conductive channels of FET were completed by thermal annealing, acid treatment, and antibody attachment. Changes associated with chemical processes were characterized by various instruments. The resulting transducers exhibited a rapid response toward cortisol molecules with accurate selectivity, stable reusability, and high sensitivity. Minimum detection level were as low as 100â¯aM with a wide linear detection range of 100â¯aM to 10â¯nM due to the large surface area of the transducer and a correspondingly high number of antibody labels. The response and applicability of these cortisol biosensors were also assessed using saliva as a test matrix.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hidrocortisona
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Carbono
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Técnicas Biossensoriais
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Nanofibras
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article