Site-specific, covalent immobilization of an engineered enterokinase onto magnetic nanoparticles through transglutaminase-catalyzed bioconjugation.

Wang, Jing-Hong; Tang, Ming-Ze; Yu, Xiao-Tian; Xu, Chong-Mei; Yang, Hong-Ming; Tang, Jin-Bao

Wang, Jing-Hong; Tang, Ming-Ze; Yu, Xiao-Tian; Xu, Chong-Mei; Yang, Hong-Ming; Tang, Jin-Bao.

Afiliação

Wang JH; School of Pharmacy, Weifang Medical University, Weifang 261053, Shandong Province, China.
Tang MZ; School of Science, China Pharmaceutical University, Nanjing 211198, Jiangsu Province, China.
Yu XT; School of Pharmacy, Weifang Medical University, Weifang 261053, Shandong Province, China.
Xu CM; School of Pharmacy, Weifang Medical University, Weifang 261053, Shandong Province, China.
Yang HM; School of Pharmacy, Weifang Medical University, Weifang 261053, Shandong Province, China. Electronic address: yanghongming2006@sohu.com.
Tang JB; School of Pharmacy, Weifang Medical University, Weifang 261053, Shandong Province, China. Electronic address: tangjb@wfmc.edu.cn.

Colloids Surf B Biointerfaces ; 177: 506-511, 2019 May 01.

Article em En | MEDLINE | ID: mdl-30818243

Assuntos

Biocatálise; Enteropeptidase/química; Enzimas Imobilizadas/metabolismo; Nanopartículas de Magnetita/química; Engenharia de Proteínas; Transglutaminases/metabolismo; Enteropeptidase/metabolismo; Enzimas Imobilizadas/química; Modelos Moleculares; Tamanho da Partícula; Especificidade por Substrato; Propriedades de Superfície; Transglutaminases/química

Palavras-chave

Engineered enterokinase; Enzymatic cleavage; Microbial transglutaminase; Reusability; Site-specific immobilization

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Enteropeptidase / Engenharia de Proteínas / Transglutaminases / Enzimas Imobilizadas / Biocatálise / Nanopartículas de Magnetita Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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