Marmoricola mangrovicus sp. nov., an endophytic actinobacterium isolated from Kandelia candel.
Int J Syst Evol Microbiol
; 69(5): 1343-1349, 2019 May.
Article
em En
| MEDLINE
| ID: mdl-30821673
ABSTRACT
A Gram-stain-positive, aerobic, motile, non-spore-forming and rod-shaped actinobacterium, designated strain 4Q3S-7T, was isolated from a piece of surface-sterilized bark of Kandelia candel collected at the Cotai Ecological Zone in Macao, China. Colonies were yellowish white, circular, smooth and convex. The 16S rRNA gene sequence of strain 4Q3S-7T exhibited highest similarities to Marmoricola ginsengisoli Gsoil 097T (97.6â%), Marmoricola solisilvae KIS18-7T (97.6â%) and Marmoricola pocheonensis Gsoil 818T (97.3â%). Phylogenetic analysis showed that strain 4Q3S-7T clustered with species of the genus Marmoricola and was clearly affiliated to the genus Marmoricola. Genomic analyses, including average nucleotide identity and DNA-DNA hybridization, clearly separated strain 4Q3S-7T from M. ginsengisoli Gsoil 097T, M. solisilvae KIS18-7T and M. pocheonensis Gsoil 818T with values below the thresholds for species delineation. Strain 4Q3S-7T had ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell wall. The major fatty acids (>10â% of total fatty acids) were C18ââ0 10-methyl (TBSA), C18ââ1ω9c, iso-C16ââ0 and iso-C16ââ0 2-OH. The predominant menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and an unidentified phospholipid. The DNA G+C content of strain 4Q3S-7T was 72.0 mol% (draft genome sequence). Based on its phylogenetic, phenotypic and chemotaxonomic features, strain 4Q3S-7T is considered to represent a novel species of the genus Marmoricola, for which the name Marmoricola mangrovicus sp. nov. is proposed. The type strain is 4Q3S-7T (=KCTC 39790T=CGMCC 4.7424T).
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MEDLINE
Assunto principal:
Filogenia
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Actinobacteria
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Casca de Planta
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Rhizophoraceae
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article