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Quenching by sodium thiosulfate does not influence 16S rRNA gene sequencing profiles of reclaimed water from three sites in the Mid-Atlantic, United States.
Allard, Sarah M; Solaiman, Sultana; Callahan, Mary Theresa; Bui, Anthony; Craddock, Hillary; Haymaker, Joseph; Foust, Derek; Duncan, Rico; Smyth, Eoghan; Mongodin, Emmanuel F; Hashem, Fawzy; May, Eric; Micallef, Shirley A; Sapkota, Amy R.
Afiliação
  • Allard SM; Maryland Institute for Applied Environmental Health, University of Maryland School of Public Health, College Park, MD, United States.
  • Solaiman S; Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, United States.
  • Callahan MT; Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, United States.
  • Bui A; Maryland Institute for Applied Environmental Health, University of Maryland School of Public Health, College Park, MD, United States.
  • Craddock H; Maryland Institute for Applied Environmental Health, University of Maryland School of Public Health, College Park, MD, United States.
  • Haymaker J; Department of Agriculture, Food, and Resource Sciences, University of Maryland Eastern Shore, Princess Anne, MD, United States.
  • Foust D; Department of Agriculture, Food, and Resource Sciences, University of Maryland Eastern Shore, Princess Anne, MD, United States.
  • Duncan R; Department of Agriculture, Food, and Resource Sciences, University of Maryland Eastern Shore, Princess Anne, MD, United States.
  • Smyth E; Maryland Institute for Applied Environmental Health, University of Maryland School of Public Health, College Park, MD, United States.
  • Mongodin EF; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, United States.
  • Hashem F; Department of Agriculture, Food, and Resource Sciences, University of Maryland Eastern Shore, Princess Anne, MD, United States.
  • May E; Department of Agriculture, Food, and Resource Sciences, University of Maryland Eastern Shore, Princess Anne, MD, United States.
  • Micallef SA; Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, United States; Center for Food Safety and Security Systems, University of Maryland, College Park, MD, United States.
  • Sapkota AR; Maryland Institute for Applied Environmental Health, University of Maryland School of Public Health, College Park, MD, United States. Electronic address: ars@umd.edu.
Environ Res ; 172: 296-300, 2019 05.
Article em En | MEDLINE | ID: mdl-30822563
A quenching agent is commonly added to chlorinated, reclaimed water during sample collection to prevent chlorine-mediated die-off of viable microbiota. However, the effect of quenching on downstream 16S rRNA-based bacterial community analyses is unclear. We conducted a side-by-side comparison of 16S rRNA sequencing data from reclaimed water samples quenched with sodium thiosulfate and non-quenched samples. Our data showed that 16 S rRNA processing and sequencing methods, and resulting bacterial profiles, were not negatively impacted by quenching.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tiossulfatos / Microbiologia da Água / Microbiota País/Região como assunto: America do norte Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tiossulfatos / Microbiologia da Água / Microbiota País/Região como assunto: America do norte Idioma: En Ano de publicação: 2019 Tipo de documento: Article