Protein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation.
Mol Cell Proteomics
; 18(5): 1027-1035, 2019 05.
Article
em En
| MEDLINE
| ID: mdl-30833379
ABSTRACT
Universal proteomics sample preparation is challenging because of the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for nonspecific immobilization on microparticles by protein aggregation capture. To demonstrate the general applicability of this mechanism, we analyzed phosphoproteomes, tissue proteomes, and interaction proteomes as well as dilute secretomes. The findings present a practical, sensitive and cost-effective proteomics sample preparation method.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteômica
/
Micropartículas Derivadas de Células
/
Agregados Proteicos
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article