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Multidimensional approach to formulating a specialized diet for northern corn rootworm larvae.
Huynh, Man P; Hibbard, Bruce E; Lapointe, Stephen L; Niedz, Randall P; French, B Wade; Pereira, Adriano E; Finke, Deborah L; Shelby, Kent S; Coudron, Thomas A.
Afiliação
  • Huynh MP; Division of Plant Sciences, University of Missouri, Columbia, Missouri, 65211, USA. mphd32@missouri.edu.
  • Hibbard BE; Plant Genetics Research Unit, USDA-Agricultural Research Service, Columbia, Missouri, 65211, USA.
  • Lapointe SL; U.S. Horticultural Research Laboratory, USDA-Agricultural Research Service, Fort Pierce, Florida, 34945, USA.
  • Niedz RP; U.S. Horticultural Research Laboratory, USDA-Agricultural Research Service, Fort Pierce, Florida, 34945, USA.
  • French BW; North Central Agricultural Research Laboratory, USDA-Agricultural Research Service, Brookings, South Dakota, 57006, USA.
  • Pereira AE; Plant Genetics Research Unit, USDA-Agricultural Research Service, Columbia, Missouri, 65211, USA.
  • Finke DL; Division of Plant Sciences, University of Missouri, Columbia, Missouri, 65211, USA.
  • Shelby KS; Biological Control of Insects Research Laboratory, USDA-Agricultural Research Service, Columbia, Missouri, 65203, USA.
  • Coudron TA; Biological Control of Insects Research Laboratory, USDA-Agricultural Research Service, Columbia, Missouri, 65203, USA.
Sci Rep ; 9(1): 3709, 2019 03 06.
Article em En | MEDLINE | ID: mdl-30842452
The northern corn rootworm (NCR), Diabrotica barberi Smith & Lawrence, is a major pest of maize (Zea mays L.). This pest has developed resistance to insecticides and adapted to crop rotation and may already be in the early stages of adaptation to toxins produced by Bacillus thuringiensis (Bt). Toxicity bioassays using artificial diet have proven to be valuable for monitoring resistance in many species, but no artificial diet has been developed specifically for NCR larvae. Toward this end, we first evaluated known Diabrotica diets to identify a starting media. We then developed a specialized diet for NCR using an iterative approach. Screening designs including 8 diet components were performed to identify the principal nutritional components contributing to multiple developmental parameters (survival, weight, and molting). We then applied mixture designs coupled with response surface modeling to optimize a blend of those components. Finally, we validated an improved NCR diet formulation that supports approximately 97% survival and molting, and a 150% increase in larval weight after 10 days of feeding compared with the best previously published artificial diet. This formulation appears suitable for use in diet bioassays as a tool for evaluating the resistance of NCR populations to insecticides.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Besouros / Alimentos Formulados / Dieta Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Besouros / Alimentos Formulados / Dieta Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article