Cell cycle- and genomic distance-dependent dynamics of a discrete chromosomal region.
J Cell Biol
; 218(5): 1467-1477, 2019 05 06.
Article
em En
| MEDLINE
| ID: mdl-30846483
In contrast to the well-studied condensation and folding of chromosomes during mitosis, their dynamics during interphase are less understood. We deployed a CRISPR-based DNA imaging system to track the dynamics of genomic loci situated kilobases to megabases apart on a single chromosome. Two distinct modes of dynamics were resolved: local movements as well as ones that might reflect translational movements of the entire domain within the nucleoplasmic space. The magnitude of both of these modes of movements increased from early to late G1, whereas the translational movements were reduced in early S phase. The local fluctuations decreased slightly in early S and more markedly in mid-late S. These newly observed movements and their cell cycle dependence suggest the existence of a hitherto unrecognized compaction-relaxation dynamic of the interphase chromosome fiber, operating concurrently with changes in the extent of overall movements of loci in the 4D genome.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteossarcoma
/
Genoma Humano
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Ciclo Celular
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Núcleo Celular
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Cromossomos Humanos
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Genômica
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Mitose
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article