Chilling followed by incubation at 37 degrees C causes a reduction in NAD+ levels which can be prevented by the poly(ADP-ribose)transferase inhibitor 3-aminobenzamide.
FEBS Lett
; 204(2): 266-8, 1986 Aug 18.
Article
em En
| MEDLINE
| ID: mdl-3089840
ABSTRACT
The exposure of cells for 60 min to a serum free medium at ice temperature followed by a return to normal culture conditions (30 min at 37 degrees C) caused a dramatic decrease in NAD+ levels. This decrease in NAD+ was prevented by 3-aminobenzamide. Alkaline elution analysis of DNA from cultures that were sisters to the ones utilized for measuring cellular NAD+ content revealed an absence of DNA breakage. These data suggest that poly(ADP-ribose)transferase may be induced in conditions not involving DNA fragmentation. The induction of this enzyme could therefore represent a cellular emergency reaction and not just a response to DNA damage.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Benzamidas
/
Temperatura Corporal
/
Temperatura Baixa
/
Inibidores de Poli(ADP-Ribose) Polimerases
/
NAD
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article