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Leishmania infantum-specific IFN-γ production in stimulated blood from cats living in areas where canine leishmaniosis is endemic.
Priolo, Vito; Martínez-Orellana, Pamela; Pennisi, Maria Grazia; Masucci, Marisa; Prandi, David; Ippolito, Dorotea; Bruno, Federica; Castelli, Germano; Solano-Gallego, Laia.
Afiliação
  • Priolo V; Dipartimento di Scienze Veterinarie, Università di Messina, Messina, Italy.
  • Martínez-Orellana P; Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
  • Pennisi MG; Dipartimento di Scienze Veterinarie, Università di Messina, Messina, Italy. pennipet@unime.it.
  • Masucci M; Dipartimento di Scienze Veterinarie, Università di Messina, Messina, Italy.
  • Prandi D; Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
  • Ippolito D; Dipartimento di Scienze Veterinarie, Università di Messina, Messina, Italy.
  • Bruno F; Centro di Referenza Nazionale per la Leishmaniosi (CReNaL), Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy.
  • Castelli G; Centro di Referenza Nazionale per la Leishmaniosi (CReNaL), Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy.
  • Solano-Gallego L; Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Parasit Vectors ; 12(1): 133, 2019 Mar 26.
Article em En | MEDLINE | ID: mdl-30909952
BACKGROUND: Feline leishmaniosis caused by Leishmania infantum is considered a rare disease in endemic areas, whereas subclinical infections are common. Immune response plays a key role in driving the course of L. infantum infection in other host species; however, the feline cell-mediated immune response to L. infantum infection has not yet been investigated. The aim of this study was to determine the cell-mediated immune response specific to L. infantum by means of interferon (IFN)-γ release in whole blood assay from cats living in endemic areas (66 in Sicily and 113 in Catalonia) and to compare with antibody levels to L. infantum [enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT)], blood parasite load and retroviral infections. RESULTS: Most cats (n = 140) were L. infantum antibody negative and only 22% (n = 39) were positive. Only 9 and 2% of tested cats had a feline immunodeficency virus (FIV) infection or a feline leukemia virus (FeLV) infection, respectively. Thirty-two cats out of 179 (18%) produced IFN-γ after stimulation with L. infantum soluble antigen (LSA) while the majority of cats (93%) produced IFN-γ after stimulation with concanavalin A (ConA). Six LSA-IFN-γ-producer cats were seropositive (three to ELISA and five to IFAT) but they were polymerase chain reaction (PCR) negative, while only one cat was antibody- and PCR-positive. Significant positive correlations were found between IFN-γ concentrations after stimulation with LSA and ConA, and between serology and PCR testing. No association was found between FIV status and LSA or ConA-IFN-γ production. Combining PCR, serology and specific IFN-γ concentration results, we found that 36% of cats studied were exposed to L. infantum. CONCLUSIONS: As expected, cats from endemic areas produce IFN-γ after ex vivo blood stimulation with LSA and therefore are able to activate a cell-mediated adaptive immune response against the parasite that is variably associated with antibody or blood PCR positivity. The association of this assay to serological and molecular tests provides a better estimate of cat exposure to L. infantum.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças do Gato / Interferon gama / Leishmania infantum / Doenças do Cão / Leishmaniose Visceral Limite: Animals País/Região como assunto: Europa Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças do Gato / Interferon gama / Leishmania infantum / Doenças do Cão / Leishmaniose Visceral Limite: Animals País/Região como assunto: Europa Idioma: En Ano de publicação: 2019 Tipo de documento: Article