Biochemical characterization of a thermostable DNA ligase from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5.

Shi, Haoqiang; Huang, Yanchao; Gan, Qi; Rui, Mianwen; Chen, Hongxun; Tu, Chuandeng; Yang, Zhihui; Oger, Philippe; Zhang, Likui

Shi, Haoqiang; Huang, Yanchao; Gan, Qi; Rui, Mianwen; Chen, Hongxun; Tu, Chuandeng; Yang, Zhihui; Oger, Philippe; Zhang, Likui.

Afiliação

Shi H; Department of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, Yangzhou, China.
Huang Y; Department of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, Yangzhou, China.
Gan Q; Department of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, Yangzhou, China.
Rui M; Department of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, Yangzhou, China.
Chen H; Department of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, Yangzhou, China.
Tu C; Department of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, Yangzhou, China.
Yang Z; College of Plant Protection, Agricultural University of Hebei, Baoding City, 071001, Hebei Province, China. bdyzh@hebau.edu.cn.
Oger P; INSA de Lyon, CNRS UMR 5240, Université de Lyon, Lyon, France. philippe.oger@insa-lyon.fr.
Zhang L; Department of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, Yangzhou, China. lkzhang@yzu.edu.cn.

Appl Microbiol Biotechnol ; 103(9): 3795-3806, 2019 May.

Article em En | MEDLINE | ID: mdl-30919103

Assuntos

Proteínas Arqueais/química; DNA Ligases/química; Thermococcus/enzimologia; Proteínas Arqueais/genética; Proteínas Arqueais/metabolismo; Clonagem Molecular; DNA/genética; DNA/metabolismo; DNA Ligases/genética; DNA Ligases/metabolismo; Estabilidade Enzimática; Temperatura Alta; Concentração de Íons de Hidrogênio; Especificidade por Substrato; Thermococcus/química; Thermococcus/genética

Palavras-chave

DNA ligase; Ligation cycling reaction; Nucleotide cofactor; Thermo-tolerance; Thermococcus barophilus

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Ligases / Thermococcus / Proteínas Arqueais Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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