Soluble siglec-5 is a novel salivary biomarker for primary Sjogren's syndrome.
J Autoimmun
; 100: 114-119, 2019 06.
Article
em En
| MEDLINE
| ID: mdl-30922727
ABSTRACT
Despite advances in the understanding of the pathogenesis, disease-specific biomarkers have not been included in the classification criteria for Primary Sjogren's syndrome (pSS). Based on a microarray of peripheral blood mononuclear cells (PBMCs) from patients with primary Sjogren's syndrome (pSS), we aimed to investigate whether soluble sialic acid-binding immunoglobulin-like lectin (siglec)-5 in saliva might be a biomarker for pSS. The concentration of siglec-5 in saliva and sera was determined by ELISA. Clinical parameters related with pSS were obtained from pSS registry and correlation with salivary siglec-5 level was evaluated. Receiver operating curve (ROC) analysis was performed to determine cut off value. A separate validation cohort consisted of subjects with suspicious pSS was evaluated to determine the performance. The level of salivary siglec-5 was significantly higher in pSS patients (nâ¯=â¯170) compared with HCs (nâ¯=â¯25), non SS sicca patients (nâ¯=â¯78) or patients with systemic lupus erythematosus (SLE) (nâ¯=â¯43) (1346.8 [202.8-4280.0] pg/mL, 6.08 [0-134.0] pg/mL, 195 [0-947.5] pg/mL, and 0 [0-238.7] pg/mL, median [interquartile range], Pâ¯<â¯0.001). Salivary siglec-5 level negatively correlated with salivary flow rate (spearman's rho -0.420, Pâ¯<â¯0.001), and positively correlated with ocular surface score (rho 0.331, Pâ¯<â¯0.001) and serum immunoglobulin G level (rhoâ¯=â¯0.202, Pâ¯=â¯0.008). In ROC analysis, area under the curve was 0.774[0.724-0.826]. With a cut off value of 400â¯pg/mL, sensitivity and specificity was 0.69 and 0.70 respectively. In validation cohort (45â¯pSS patients and 45 non SS sicca patients), sensitivity and specificity of siglec-5 was 64.4% and 77.8%, respectively. In conclusion, the level of soluble siglec-5 is significantly higher in the saliva from pSS patients, which reflects the severity of hyposalivation and ocular surface damage. This novel salivary biomarker may provide benefits for pSS diagnosis.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saliva
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Antígenos de Diferenciação Mielomonocítica
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Antígenos CD
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Lectinas
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article