Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane.
Int J Biol Macromol
; 132: 615-628, 2019 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-30940588
ABSTRACT
In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1-1.0â¯M), chitosan coupling pH (6-8), chitosan coupling concentration (0.1-3.0%), algal solution pH (6-8), algal mass concentration (0.1-1.0% dw/v), and membrane flux (4.08â¯×â¯10-2-2.04â¯×â¯10-1â¯mL/min·cm2) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP)â¯>â¯allophycocyanin (APC)â¯>â¯CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ficocianina
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Cromatografia
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Quitosana
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Nanofibras
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Membranas Artificiais
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article