Bayesian Detection of Abnormal Asynchrony of Division Between Sister Cells in Mutant Caenorhabditis elegans Embryos.
J Comput Biol
; 26(5): 495-505, 2019 05.
Article
em En
| MEDLINE
| ID: mdl-30964328
ABSTRACT
Cell division timing is critical for cell fate specification and morphogenesis during embryogenesis, but how division timings are regulated among cells during development is poorly understood. In this article, we focus on the comparison of asynchrony of division, that is, difference of lifetime, between sister cells (ADS) among wild-type and mutant individuals of Caenorhabditis elegans. On the one hand, due to extreme imbalance between wild-type individuals and mutant-type samples, direct comparison of two distributions of ADS between wild type and mutant type is not feasible. On the other hand, we originally found that the ADS is correlated with the lifespan of the corresponding mother cell in wild type. Hence, a semiparametric Bayesian quantile regression method where lifetime of the mother cell is taken as covariate is developed to estimate the 95% confidence curve of ADS in wild type and then ADS of mutant type is classified as abnormal if outside the corresponding confidence interval. A high accuracy of our method is demonstrated by a large-scale simulation study. Real data analysis shows that ADS is related to gene function and expression quantitatively.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Divisão Celular
/
Caenorhabditis elegans
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Embrião não Mamífero
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Mutação
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article