Synthesis of Erythropoietins Site-Specifically Conjugated with Complex-Type N-Glycans.
Chembiochem
; 20(15): 1914-1918, 2019 08 01.
Article
em En
| MEDLINE
| ID: mdl-30973186
ABSTRACT
The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans. We aimed to readily attach defined N-glycans to EPO through copper-catalyzed azide alkyne cycloaddition. EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding. Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Eritropoetina
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article