Generation of induced pluripotent stem cells (iPSCs) IRMBi002-A from an Alzheimer's disease patient carrying a D694N mutation in the APP gene.
Stem Cell Res
; 37: 101438, 2019 05.
Article
em En
| MEDLINE
| ID: mdl-31004935
ABSTRACT
Induced pluripotent stem cells (iPSC) were generated from skin fibroblasts obtained from a 58â¯year-old woman suffering from Alzheimer's disease and carrying a D694N mutation on Amyloid precursor protein (APP). Fibroblasts were reprogrammed into iPSC using the integration-free Sendai Virus which allows the expression of the Yamanaka factors. Verification of their pluripotency was achieved by demonstrating the expression of pluripotency markers and their differentiation potential into the three primary germ layers. The cells have the corresponding mutation and present a normal karyotype. The reported APP-D694N iPSC line may be used to model and study human AD pathology in vitro.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
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Precursor de Proteína beta-Amiloide
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Reprogramação Celular
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Células-Tronco Pluripotentes Induzidas
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Doença de Alzheimer
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Fibroblastos
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Mutação
Tipo de estudo:
Prognostic_studies
Limite:
Female
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Humans
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Middle aged
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article