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Identification of new loci for salt tolerance in soybean by high-resolution genome-wide association mapping.
Do, Tuyen D; Vuong, Tri D; Dunn, David; Clubb, Michael; Valliyodan, Babu; Patil, Gunvant; Chen, Pengyin; Xu, Dong; Nguyen, Henry T; Shannon, J Grover.
Afiliação
  • Do TD; Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA.
  • Vuong TD; Present address: The Cuu Long Delta Rice Research Institute, Thoi Lai District, Can Tho City, Vietnam.
  • Dunn D; Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA.
  • Clubb M; Division of Plant Sciences, University of Missouri, Portageville, MO, 63873, USA.
  • Valliyodan B; Division of Plant Sciences, University of Missouri, Portageville, MO, 63873, USA.
  • Patil G; Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA.
  • Chen P; Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA.
  • Xu D; Present Address: Department Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN, 55108, USA.
  • Nguyen HT; Division of Plant Sciences, University of Missouri, Portageville, MO, 63873, USA.
  • Shannon JG; Department of Electric Engineering and Computer Science, Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO, 65211, USA.
BMC Genomics ; 20(1): 318, 2019 Apr 25.
Article em En | MEDLINE | ID: mdl-31023240
BACKGROUND: Salinity is an abiotic stress that negatively affects soybean [Glycine max (L.) Merr.] seed yield. Although a major gene for salt tolerance was identified and consistently mapped to chromosome (Chr.) 3 by linkage mapping studies, it does not fully explain genetic variability for tolerance in soybean germplasm. In this study, a genome-wide association study (GWAS) was performed to map genomic regions for salt tolerance in a diverse panel of 305 soybean accessions using a single nucleotide polymorphism (SNP) dataset derived from the SoySNP50K iSelect BeadChip. A second GWAS was also conducted in a subset of 234 accessions using another 3.7 M SNP dataset derived from a whole-genome resequencing (WGRS) study. In addition, three gene-based markers (GBM) of the known gene, Glyma03g32900, on Chr. 3 were also integrated into the two datasets. Salt tolerance among soybean lines was evaluated by leaf scorch score (LSS), chlorophyll content ratio (CCR), leaf sodium content (LSC), and leaf chloride content (LCC). RESULTS: For both association studies, a major locus for salt tolerance on Chr. 3 was confirmed by a number of significant SNPs, of which three gene-based SNP markers, Salt-20, Salt14056 and Salt11655, had the highest association with all four traits studied. Also, additional genomic regions on Chrs. 1, 8, and 18 were found to be associated with various traits measured in the second GWAS using the WGRS-derived SNP dataset. CONCLUSIONS: A region identified on Chr. 8 was identified to be associated with all four traits and predicted as a new minor locus for salt tolerance in soybean. The candidate genes harbored in this minor locus may help reveal the molecular mechanism involved in salt tolerance and to improve tolerance in soybean cultivars. The significant SNPs will be useful for marker-assisted selection for salt tolerance in soybean breeding programs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glycine max / Estudo de Associação Genômica Ampla / Tolerância ao Sal Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glycine max / Estudo de Associação Genômica Ampla / Tolerância ao Sal Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article