Label-Free Detection of DNA Polymerase Activity Using Hairpin DNA-Templated Silver Nanoclusters.
J Nanosci Nanotechnol
; 19(10): 6534-6538, 2019 10 01.
Article
em En
| MEDLINE
| ID: mdl-31026989
ABSTRACT
Based on the characteristic properties like strong fluorescence and high photostability, DNA-templated silver nanoclusters (DNA-AgNCs) were utilized to detect Klenow fragment exo- (KF-) activity and to monitor its inhibition by daidzein. In this study, fluorescence intensity is linearly correlated for KF- concentration ranging from 0 to 2.0 U/mL, with a minimum detectable concentration of 0.05 U/mL. In addition, KF- inhibition was monitored by changes in fluorescence intensity with increasing daidzein concentration gradually.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Nanopartículas Metálicas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article