Electrical pulse stimulation of skeletal myoblasts cell cultures with simulated action potentials.
J Tissue Eng Regen Med
; 13(7): 1265-1269, 2019 07.
Article
em En
| MEDLINE
| ID: mdl-31033223
ABSTRACT
Electrical pulse stimulation has an important effect on skeletal muscle development and maturation. However, the methodology for controlling these stimulation parameters to develop in vitro functional skeletal muscle tissues remains to be established. In this work, we have studied the effect of simulated action potentials on the growth and differentiation of skeletal myoblast cell cultures. A circuit simulating action potentials of 0.15 and 0.3 V/mm, at a frequency of 1 Hz and with a 4-ms pulse width, is proposed. Results show an important improvement of the growth rate and differentiation of myoblasts at a voltage of 0.15 V/mm. Parameters such as electrodes geometry or type of signals must be considered in the development of in vitro skeletal muscle.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Potenciais de Ação
/
Desenvolvimento Muscular
/
Mioblastos Esqueléticos
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article