Polymorphism rs7895833 in the SIRT1 gene and its association with dyslipidaemia in the elderly.

ABSTRACT

OBJECTIVES: Sirtuin 1 is a human protein involved in gene silencing and in inducing the deacetylation of proteins involved in the metabolic and adaptive response mechanisms. Polymorphisms in the SIRT1 gene have been studied with respect to aging. This study aims to determine the allelic and genotypic frequencies of the rs7895833 A/G polymorphism in the SIRT1 gene, and to identify the association between this polymorphism and the co-morbidities prevalent in the elderly population. MATERIAL AND METHODS: A total of 216 patients were evaluated in an outpatient clinic in Central Brazil. The individuals underwent validated tests for cognitive impairment and falls risk, serum biochemistry analysis, as well as polymer chain reaction (PCR) with confronting two-pair primers for polymorphism genotyping. RESULTS: rs7895833 polymorphism in SIRT1 gene was observed in these patients as follows AA (56/216), AG (138/216), and GG (22/216). The frequency of allele A was 0.58, and that of allele G was 0.42. In the multivariate analysis of the exploratory variables, glucose, high density lipoprotein (HDL) cholesterol, systemic arterial hypertension, dyslipidaemia, and depression, which were associated in the univariate analysis with the polymorphism rs7895833, only dyslipidaemia showed a statistically significant difference in a greater number of individuals with this polymorphism. CONCLUSION: The variant allele G of the SIRT1 gene polymorphism was found in 42% of these Brazilian geriatric patients, and was associated with dyslipidaemia. Further studies should be performed to confirm this result and to elucidate the role of SIRT1 in lipid metabolism.