Antibody Purification and Storage.
Cold Spring Harb Protoc
; 2019(5)2019 05 01.
Article
em En
| MEDLINE
| ID: mdl-31043563
ABSTRACT
Antibodies have become a common and necessary tool in biochemistry, cell biology, and immunology laboratories. There are many different types of antibodies and antibody fragments being used for a myriad of applications. As a result, many different purification protocols have been developed to obtain antibodies of the desired specificity and sensitivity. Here, we introduce the options for small- to large-scale antibody purification and isolation of polyclonal and monoclonal antibodies (and fragments generated from these) that target-specific proteins, as well as methods to properly purify antibodies that recognize posttranslational modifications. Optimal conditions for the long-term storage of antibodies are also discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anticorpos
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article