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Evaluation of equine articular cartilage degeneration after mechanical impact injury using cationic contrast-enhanced computed tomography.
Nelson, B B; Mäkelä, J T A; Lawson, T B; Patwa, A N; Barrett, M F; McIlwraith, C W; Hurtig, M B; Snyder, B D; Moorman, V J; Grinstaff, M W; Goodrich, L R; Kawcak, C E.
Afiliação
  • Nelson BB; Equine Orthopaedic Research Center, Colorado State University, Fort Collins, CO, USA.
  • Mäkelä JTA; Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA; Department of Chemistry, Boston University, Boston, MA, USA.
  • Lawson TB; Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA; Department of Mechanical Engineering, Boston University, Boston, MA, USA.
  • Patwa AN; Department of Chemistry, Boston University, Boston, MA, USA; SLSE (Chemistry), Navrachana University, Vadodara, Gujarat, India.
  • Barrett MF; Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA.
  • McIlwraith CW; Equine Orthopaedic Research Center, Colorado State University, Fort Collins, CO, USA.
  • Hurtig MB; Department of Clinical Studies, University of Guelph, Ontario, Canada.
  • Snyder BD; Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
  • Moorman VJ; Equine Orthopaedic Research Center, Colorado State University, Fort Collins, CO, USA.
  • Grinstaff MW; Department of Chemistry, Boston University, Boston, MA, USA; Department of Mechanical Engineering, Boston University, Boston, MA, USA; Departments of Biomedical Engineering, and Medicine, Boston University, Boston, MA, USA.
  • Goodrich LR; Equine Orthopaedic Research Center, Colorado State University, Fort Collins, CO, USA.
  • Kawcak CE; Equine Orthopaedic Research Center, Colorado State University, Fort Collins, CO, USA. Electronic address: ckawcak@colostate.edu.
Osteoarthritis Cartilage ; 27(8): 1219-1228, 2019 08.
Article em En | MEDLINE | ID: mdl-31075424
ABSTRACT

OBJECTIVE:

Cationic agent contrast-enhanced computed tomography (cationic CECT) characterizes articular cartilage ex vivo, however, its capacity to detect post-traumatic injury is unknown. The study objectives were to correlate cationic CECT attenuation with biochemical, mechanical and histological properties of cartilage and morphologic computed tomography (CT) measures of bone, and to determine the ability of cationic CECT to distinguish subtly damaged from normal cartilage in an in vivo equine model.

DESIGN:

Mechanical impact injury was initiated in equine femoropatellar joints in vivo to establish subtle cartilage degeneration with site-matched controls. Cationic CECT was performed in vivo (clinical) and postmortem (microCT). Articular cartilage was characterized by glycosaminoglycan (GAG) content, biochemical moduli and histological scores. Bone was characterized by volume density (BV/TV) and trabecular number (Tb.N.), thickness (Tb.Th.) and spacing (Tb.Sp.).

RESULTS:

Cationic CECT attenuation (microCT) of cartilage correlated with GAG (r = 0.74, P < 0.0001), compressive modulus (Eeq) (r = 0.79, P < 0.0001) and safranin-O histological score (r = -0.66, P < 0.0001) of cartilage, and correlated with BV/TV (r = 0.37, P = 0.0005), Tb.N. (r = 0.39, P = 0.0003), Tb.Th. (r = 0.28, P = 0.0095) and Tb.Sp. (r = -0.44, P < 0.0001) of bone. Mean [95% CI] cationic CECT attenuation at the impact site (2215 [1987, 2443] Hounsfield Units [HUs]) was lower than site-matched controls (2836 [2490, 3182] HUs, P = 0.036). Clinical cationic CECT attenuation correlated with GAG (r = 0.23, P = 0.049), Eeq (r = 0.26, P = 0.025) and safranin-O histology score (r = -0.32, P = 0.0046).

CONCLUSIONS:

Cationic CECT (microCT) reflects articular cartilage properties enabling segregation of subtly degenerated from healthy tissue and also reflects bone morphometric properties on CT. Cationic CECT is capable of characterizing articular cartilage in clinical scanners.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cartilagem Articular / Microtomografia por Raio-X Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cartilagem Articular / Microtomografia por Raio-X Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article