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Cryopreservation and hormonal induction of spermic urine in a novel species: The smooth-sided toad (Rhaebo guttatus).
Hinkson, Kristin M; Baecher, J Alex; Poo, Sinlan.
Afiliação
  • Hinkson KM; Memphis Zoo, Department of Research and Conservation, 2000 Prentiss Place, Memphis, TN, 38112, USA. Electronic address: kmhinkson14@gmail.com.
  • Baecher JA; Memphis Zoo, Department of Research and Conservation, 2000 Prentiss Place, Memphis, TN, 38112, USA. Electronic address: jbaecher@gmail.com.
  • Poo S; Memphis Zoo, Department of Research and Conservation, 2000 Prentiss Place, Memphis, TN, 38112, USA. Electronic address: sheilapoo@gmail.com.
Cryobiology ; 89: 109-111, 2019 08.
Article em En | MEDLINE | ID: mdl-31078579
Global amphibian declines have fueled an increased interest in amphibian assisted reproductive technologies. Within the genus Rhaebo, half of the species are experiencing decreasing population trends; however, insufficient information is available on many of these species' reproductive biology. Using the smooth-sided toad, Rhaebo guttatus, we present effective methods for collecting and cryopreserving an example of Rhaebo sperm. Specifically, our findings show that administering 10 IU/g body weight of hCG (human chorionic gonadotropin) yields the most motile and concentrated sperm and that cryopreserving spermic urine in a solution of 5% DMFA (N,N-Dimethylformamide) and 10% trehalose returns sperm with a 33 ±â€¯3% average post-thaw motility. These findings may represent an important step forward in developing techniques that can be safely applied to other, more vulnerable species within the Rhaebo genus.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Bufonidae / Criopreservação / Crioprotetores / Gonadotropina Coriônica Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Bufonidae / Criopreservação / Crioprotetores / Gonadotropina Coriônica Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2019 Tipo de documento: Article